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Western blot analysis in HL-60 cell line lysates (35ug/lane).
TRIM23 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

TRIM23 Antibody

CATALOG NUMBER: 57-172

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This TRIM23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human TRIM23.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM23
Additional Names:
E3 ubiquitin-protein ligase TRIM23, 632-, ADP-ribosylation factor domain-containing protein 1, GTP-binding protein ARD-1, RING finger protein 46, Tripartite motif-containing protein 23, TRIM23, ARD1, ARFD1, RNF46
Protein Accession Number:
P36406
Protein Gi Number:
543839
Ncbi Gene Id Number:
373
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thetripartite motif (TRIM) family. The TRIM motif includes threezinc-binding domains, a RING, a B-box type 1 and a B-box type 2,and a coiled-coil region. This protein is also a member of the ADPribosylation factor family of guanine nucleotide-binding family ofproteins. Its carboxy terminus contains an ADP-ribosylation factordomain and a guanine nucleotide binding site, while the aminoterminus contains a GTPase activating protein domain which acts onthe guanine nucleotide binding site. The protein localizes tolysosomes and the Golgi apparatus. It plays a role in the formationof intracellular transport vesicles, their movement from onecompartment to another, and phopholipase D activation. Threealternatively spliced transcript variants for this gene have beendescribed.
Background Reference 1:
Arimoto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 107(36):15856-15861(2010)
Background Reference 2:
Poole, E., et al. J. Virol. 83(8):3581-3590(2009)
Background Reference 3:
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Background Reference 4:
Vichi, A., et al. Proc. Natl. Acad. Sci. U.S.A. 102(6):1945-1950(2005)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-172

List Size:
400 ul

List Price:

$495.00

Datasheet
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