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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

TRIM27 Antibody

CATALOG NUMBER: 58-074

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TRIM27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-482 amino acids from the C-terminal region of human TRIM27.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM27
Additional Names:
Zinc finger protein RFP, 632-, RING finger protein 76, Ret finger protein, Tripartite motif-containing protein 27, TRIM27, RFP, RNF76
Protein Accession Number:
P14373
Protein Gi Number:
132517
Ncbi Gene Id Number:
5987
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the tripartite motif (TRIM)family. The TRIM motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region. Thisprotein localizes to the nuclear matrix. It interacts with theenhancer of polycomb protein and represses gene transcription. Itis also thought to be involved in the differentiation of male germcells. Fusion of the N-terminus of this protein with the truncatedC-terminus of the RET gene product has been shown to result inproduction of the ret transforming protein.
Background Reference 1:
Johnson, A.D., et al. Nat. Genet. 42(7):608-613(2010)
Background Reference 2:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) :
Background Reference 3:
Tezel, G.G., et al. Pathol. Res. Pract. 205(6):403-408(2009)
Background Reference 4:
Li, X., et al. Virology 360(2):419-433(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-074

List Size:
400 ul

List Price:

$495.00

Datasheet
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