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46-525 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
46-525 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TRIM4 Antibody

CATALOG NUMBER: 46-525

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ASLVIPPVTDRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55-60kDa band observed in HepG2 whole cell lysate (predicted MW of 58kDa and 55kDa according to NP_148977 and NP_149082). Recommended for use at 1ug/ml.
Specificity:
This antibody is expected to recognise both reported human isoforms (as represented by NP_148977 and NP_149082).
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRIM4
Additional Names:
TRIM4, RNF87, tripartite motif-containing 4, tripartite motif protein 4, tripartite motif protein TRIM4, tripartite motif protein TRIM4 isoform alpha, tripartite motif protein 4
Protein Accession Number:
NP_148977.1,NP_149082.1
Protein Gi Number:
301601615
Ncbi Gene Id Number:
89122
Background Reference 1:
Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A. The tripartite motif family identifies cell compartments. EMBO J. 2001 May 1;20(9):2140-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-525

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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