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Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).
TRIM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549,293,HepG2 cell line lysates (35ug/lane).

TRIM4 Antibody

CATALOG NUMBER: 56-971

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TRIM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the N-terminal region of human TRIM4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM4
Additional Names:
E3 ubiquitin-protein ligase TRIM4, 632-, RING finger protein 87, Tripartite motif-containing protein 4, TRIM4, RNF87
Protein Accession Number:
Q9C037
Protein Gi Number:
209572691
Ncbi Gene Id Number:
89122
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thetripartite motif (TRIM) family. The TRIM motif includes threezinc-binding domains, a RING, a B-box type 1 and a B-box type 2,and a coiled-coil region. The protein localizes to cytoplasmicbodies. Its function has not been identified. Alternatively splicedtranscript variants that encode different isoforms have beendescribed.
Background Reference 1:
Kasyapa, C., et al. Proteomics 9(16):3979-3988(2009)
Background Reference 2:
Li, X., et al. Virology 360(2):419-433(2007)
Background Reference 3:
Thompson, E.E., et al. Pharmacogenomics J. 6(2):105-114(2006)
Background Reference 4:
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-971

List Size:
400 ul

List Price:

$495.00

Datasheet
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