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Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.
Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha antibody at 10 μg/mL.
Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.
Trial Size

TRIM5 alpha Antibody

CATALOG NUMBER: 3249

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.
The immunogen is located within the last 50 amino acids of TRIM5 alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Anti-TRIM5 alpha is human reactive but should presumably also react with rhesus protein.
Positive Control 1:
Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2:
Cat. No. 1317 - Human Uterus Tissue Lysate
Positive Control 3:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 4:
Cat. No. 10-001 - Human Breast Tissue Slide
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 55 kDa
Purification:
TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM5 alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM5
Additional Names:
TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
Protein Accession Number:
ABG67967
Protein Gi Number:
110339457
Ncbi Gene Id Number:
574288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
Background Reference 1:
Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
Background Reference 2:
Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
Background Reference 3:
Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3249

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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