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Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.
Immunofluorescence of TRIM5 alpha in human breast tissue with TRIM5 alpha antibody at 10 μg/mL.
Western blot analysis of TRIM5 alpha expression in (A) human stomach, (B) thymus, and (C) uterus tissue lysate with TRIM5 alpha antibody at 2 μg/ml.
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TRIM5 alpha Antibody

CATALOG NUMBER: 3249

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5 alpha.
The immunogen is located within the last 50 amino acids of TRIM5 alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. Antibody can also be used for immunoflourescence starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Anti-TRIM5 alpha is human reactive but should presumably also react with rhesus protein.
Positive Control 1:
Cat. No. 1319 - Human Stomach Tissue Lysate
Positive Control 2:
Cat. No. 1317 - Human Uterus Tissue Lysate
Positive Control 3:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 4:
Cat. No. 10-001 - Human Breast Tissue Slide
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 55 kDa
Purification:
TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TRIM5 alpha Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM5 alpha antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM5
Additional Names:
TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
Protein Accession Number:
ABG67967
Protein Gi Number:
110339457
Ncbi Gene Id Number:
574288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
Background Reference 1:
Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
Background Reference 2:
Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
Background Reference 3:
Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3249

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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