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46-526 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
46-526 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

TRIM5 Antibody

CATALOG NUMBER: 46-526

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: CNIEKNENYQPK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3kDa according to NP_149023.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.
Specificity:
This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRIM5
Additional Names:
TRIM5, tripartite motif-containing 5 , HGNC:16276, RNF88, tripartite motif protein 5, tripartite motif protein TRIM5, TRIM5alpha, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Protein Accession Number:
NP_149023.1
Protein Gi Number:
14719418
Ncbi Gene Id Number:
85363
Background Reference 1:
Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci USA. 2005 Oct 11;102(41):14849-53. Epub 2005 Oct 3.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-526

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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