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Western blot analysis in mouse stomach tissue lysates (35ug/lane).
TRIM69 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse stomach tissue lysates (35ug/lane).

TRIM69 Antibody

CATALOG NUMBER: 56-233

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This TRIM69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-129 amino acids from the N-terminal region of human TRIM69.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIM69
Additional Names:
E3 ubiquitin-protein ligase TRIM69, 632-, RFP-like domain-containing protein trimless, RING finger protein 36, Tripartite motif-containing protein 69, TRIM69, RNF36
Protein Accession Number:
Q86WT6
Protein Gi Number:
229462760
Ncbi Gene Id Number:
140691
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the RING-B-box-coiled-coil(RBCC) family and encodes a protein with an N-terminal RING fingermotif, a PRY domain and a C-terminal SPRY domain. The mouseortholog of this gene is specifically expressed in germ cells atthe round spermatid stages during spermatogenesis and, whenoverexpressed, induces apoptosis. Alternatively spliced transcriptvariants encoding distinct isoforms have been described. It may play a role in apoptosis (By similarity).
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Shyu, H.W., et al. Exp. Cell Res. 287(2):301-313(2003)
Background Reference 4:
Shyu, H.W., et al. Mech. Dev. 108 (1-2), 213-216 (2001) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-233

List Size:
400 ul

List Price:

$495.00

Datasheet
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