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42-540 (0.3ug/ml) staining of HEK293 (A) and NIH3T3 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-540 (3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-540 (0.3ug/ml) staining of HEK293 (A) and NIH3T3 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TRIM71 Antibody

CATALOG NUMBER: 42-540

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RQVKAKSLYLQVE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 100kDa band observed in lysates of the cell lines HEK293 and NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and 92.1kDa according to Mouse NP_001035968.1). The observed molecular weight corresponds to findings with EB09902, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Positive Control 2:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRIM71
Additional Names:
TRIM71, tripartite motif-containing 71, LIN-41, LIN41, abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41
Protein Accession Number:
NP_001034200.1
Protein Gi Number:
84993742
Ncbi Gene Id Number:
131405

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-540

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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