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Western blot analysis in K562 cell line lysates (35ug/lane).
Western blot analysis in K562 cell line lysates (35ug/lane).

TRIP10 Antibody

CATALOG NUMBER: 57-672

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This TRIP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the N-terminal region of human TRIP10.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIP10
Additional Names:
Cdc42-interacting protein 4, Protein Felic, Salt tolerant protein, hSTP, Thyroid receptor-interacting protein 10, TR-interacting protein 10, TRIP-10, TRIP10, CIP4, STOT, STP
Protein Accession Number:
Q15642
Protein Gi Number:
118572632
Ncbi Gene Id Number:
9322
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Background Reference 1:
Roignot, J., et al. Cancer Lett. 288(1):116-123(2010)
Background Reference 2:
Hu, J., et al. Cell. Signal. 21(11):1686-1697(2009)
Background Reference 3:
Banerjee, P.P., et al. J. Exp. Med. 204(10):2305-2320(2007)
Background Reference 4:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-672

List Size:
400 ul

List Price:

$495.00

Datasheet
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