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Western blot analysis of TRIP13 Antibody in K562, Hela, 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TRIP13 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TRIP13 using rabbit polyclonal TRIP13 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.
Western blot analysis of TRIP13 Antibody in K562, Hela, 293 cell line lysates (35ug/lane)

TRIP13 Antibody

CATALOG NUMBER: 61-545

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TRIP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-90 amino acids from the N-terminal region of human TRIP13.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIP13
Additional Names:
Pachytene checkpoint protein 2 homolog, Human papillomavirus type 16 E1 protein-binding protein, 16E1-BP, HPV16 E1 protein-binding protein, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13, TR-interacting protein 13, TRIP-13, TRIP13, PCH2
Protein Accession Number:
Q15645
Protein Gi Number:
85541056
Ncbi Gene Id Number:
9319
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIP13 specifically interacts with the ligand binding domain of the thyroid receptor (TR). This interaction does not require the presence of thyroid hormone for its interaction.
Background Reference 1:
Rush,J., Nat. Biotechnol. 23 (1), 94-101 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-545

List Size:
400 ul

List Price:

$495.00

Datasheet
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