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Western blot analysis of extracts of various cell lines, using TrkB Antibody (14-225) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human lung cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TrkB antibody (14-225) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using TrkB Antibody (14-225) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

TrkB Antibody

CATALOG NUMBER: 14-225

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-433 of human TrkB (NP_001007098.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
K-562
Positive Control 2:
Rat brain
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NTRK2
Additional Names:
NTRK2, neurotrophic tyrosine kinase, receptor, type 2, GP145-TrkB, TRKB , BDNF/NT-3 growth factors receptor, tyrosine kinase receptor B, BDNF receptor
Ncbi Gene Id Number:
4915
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-225

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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