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Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.

TRMT5 Antibody

CATALOG NUMBER: 26-357

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRMT5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TRMT5 antibody can be used for detection of TRMT5 by ELISA at 1:62500. TRMT5 antibody can be used for detection of TRMT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Predicted Molecular Weight:
58 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TRMT5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TRMT5
Additional Names:
TRMT5, KIAA1393, MGC111453, TRM5
Protein Accession Number:
NP_065861
Protein Gi Number:
145275187
Ncbi Gene Id Number:
57570
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).
Background Reference 1:
Brule, H., (2004) Biochemistry 43 (28), 9243-9255.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-357

List Size:
100 ul

List Price:

$519.00

Datasheet
Shipping Info

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