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46-736 (0.2ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-736 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-736 (0.2ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TROP2 Antibody

CATALOG NUMBER: 46-736

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-HYEQPTIQIELRQN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx. 37kDa band observed in Human Prostate lysates (calculated MW of 35.7kDa according to NP_002344.2). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 2.5ug/ml.
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TACSTD2
Additional Names:
TROP2, TACSTD2, tumor-associated calcium signal transducer 2, EGP-1, GA733, GA733-1, M1S1, epithelial glycoprotein-1, membrane component, chromosome 1, surface marker 1 (40kD glycoprotein, identified by monoclonal antibody GA733)
Protein Accession Number:
NP_002344.2
Protein Gi Number:
166795236
Ncbi Gene Id Number:
4070
Background Reference 1:
Shimada A, Kano J, Ishiyama T, Okubo C, Iijima T, Morishita Y, Minami Y, Inadome Y, Shu Y, Sugita S, Takeuchi T, Noguchi M. Establishment of an immortalized cell line from a precancerous lesion of lung adenocarcinoma, and genes highly expressed in the early stages of lung adenocarcinoma development. Cancer Sci. 2005 Oct;96(10):668-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-736

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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