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43-548 (0.03ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-548 (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-548 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-548 (0.03ug/ml) staining of Human Platelets lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

tropomyosin 4 / TPM4 Antibody

CATALOG NUMBER: 43-548

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ENVGLHQTLDQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 32kDa band observed in Human Platelets, Pig Heart and in NIH3T3 lysates (calculated MW of 32.7kDa according to NP_001138632.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Placenta and Prostate. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001138632.1; NP_003281.1).
Predicted Molecular Weight:
Approx 32kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
tropomyosin 4 / TPM4
Additional Names:
TPM4, tropomyosin 4, TM30p1, tropomyosin alpha-4 chain, tropomyosin-4
Protein Accession Number:
NP_001138632.1,NP_003281.1
Protein Gi Number:
223555975
Ncbi Gene Id Number:
7171
Background Reference 1:
Vlahovich N, Schevzov G, Nair-Shaliker V, Ilkovski B, Artap ST, Joya JE, Kee AJ, North KN, Gunning PW, Hardeman EC.Tropomyosin 4 defines novel filaments in skeletal muscle associated with muscle remodelling/regeneration in normal and diseased muscle.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-548

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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