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Antibody used in WB on Mouse SP2/0 at 2.5 ug/ml.
Antibody used in WB on Mouse at 1:1000 (Lane 1: 40ug C57/B6 control mouse G.I. Lane 2: 40ug C57/B6 mouse G.I. treated with Irinotecan Lane 3: 40ug p53 KO HCT-116 cells Lane 4: 40ug C57/B6 mouse treated with 15 Gy ionizing radiation ).
Antibody used in WB on Mouse SP2/0 at 2.5 ug/ml.

TRP53 Antibody

CATALOG NUMBER: 28-625

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse TRP53.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TRP53 antibody can be used for detection of TRP53 by ELISA at 1:312500. TRP53 antibody can be used for detection of TRP53 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
SP2/0 Cell Lysate
Predicted Molecular Weight:
43 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TRP53 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TRP53
Additional Names:
TRP53, bbl, bfy, bhy, p44, p53, Tp53
Protein Accession Number:
NP_035770
Protein Gi Number:
148747262
Ncbi Gene Id Number:
22059
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Trp53 is a protein found in elevated levels in a great variety of transformed cells
Background Reference 1:
Rotter, V., et al., (1984) Mol. Cell. Biol. 4 (2), 383-385.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-625

List Size:
100 ul

List Price:

$473.00

Datasheet
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