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Western blot analysis of extracts of mouse liver, using TRPA1 antibody (23-662) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TRPA1 antibody (23-662) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse liver, using TRPA1 antibody (23-662) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

TRPA1 Antibody

CATALOG NUMBER: 23-662

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 850-950 of human TRPA1 (NP_015628.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse liver
Predicted Molecular Weight:
Observed: 128kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TRPA1
Additional Names:
TRPA1, ANKTM1, TRPA-1
Ncbi Gene Id Number:
8989
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-662

List Size:
100 uL

List Price:

$436.00

Datasheet
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