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Western blot analysis in HL-60 cell line lysates (35ug/lane).
TRPC5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

TRPC5 Antibody

CATALOG NUMBER: 57-257

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rabbit
Immunogen:
This TRPC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-283 amino acids from the N-terminal region of human TRPC5.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
111 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRPC5
Additional Names:
Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5, TRPC5, TRP5
Protein Accession Number:
Q9UL62
Protein Gi Number:
10720321
Ncbi Gene Id Number:
7224
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the transient receptor family. Itencodes one of the seven mammalian TRPC (transient receptorpotential channel) proteins. The encoded protein is a multi-passmembrane protein and is thought to form a receptor-activatednon-selective calcium permeant cation channel. The protein isactive alone or as a heteromultimeric assembly with TRPC1, TRPC3,and TRPC4. It also interacts with multiple proteins includingcalmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor(NHERF ), interferon-induced GTP-binding protein (MX1), ring fingerprotein 24 (RNF24), and SEC14 domain and spectrin repeat-containingprotein 1 (SESTD1).
Background Reference 1:
Al-Shawaf, E., et al. Arterioscler. Thromb. Vasc. Biol. 30(7):1453-1459(2010)
Background Reference 2:
Wong, C.O., et al. Pflugers Arch. 460(1):121-130(2010)
Background Reference 3:
Miehe, S., et al. J. Biol. Chem. 285(16):12426-12434(2010)
Background Reference 4:
Gross, S.A., et al. J. Biol. Chem. 284(49):34423-34432(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-257

List Size:
400 ul

List Price:

$495.00

Datasheet
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