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Western blot analysis of TRPC6 in K562 cell lysate with TRPC6 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TRPC6 in mouse lung tissue with TRPC6 antibody at 10 μg/mL.
Immunocystochemistry of TRPC6 in K562 cells with TRPC6 antibody at 2 μg/mL.
Immunofluorescence of TRPC6 in K562 cells with TRPC6 antibody at 5 μg/ml.<br><br>Red: TRPC6 Antibody (3899) <br> Blue: DAPI staining
Western blot analysis of TRPC6 in K562 cell lysate with TRPC6 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TRPC6 Antibody

CATALOG NUMBER: 3899

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TRPC6 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human TRPC6.
The immunogen is located within amino acids 50 - 100 of TRPC6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRPC6 antibody can be used for the detection of TRPC6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for Immunohistochemistry starting at 10 μg/mL and Immunocytochemistry starting at 2 μg/mL . For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This antibody may cross-react with TRPC3.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Purification:
TRPC6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRPC6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRPC6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRPC6
Additional Names:
TRPC6 Antibody: TRP6, FSGS2, TRP6, Short transient receptor potential channel 6, Transient receptor protein 6, TrpC6
Protein Accession Number:
AAH93658
Protein Gi Number:
62739910
Ncbi Gene Id Number:
7225
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRPC6 Antibody: The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, TRPC6 acts as a non-selective store-independent receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that TRPC6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.
Background Reference 1:
Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
Background Reference 2:
Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
Background Reference 3:
Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3899

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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