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46-531 (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
46-531 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments
46-531 (4ug/ml) staining of paraffin embedded Mouse Hippocampus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

TRPM7 Antibody

CATALOG NUMBER: 46-531

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: C-TKESESTNSVRLML
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Mouse Brain shows neuronal staining in the Hippocampus. Recommended concentration: 4-6ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRPM7
Additional Names:
TRPM7, transient receptor potential cation channel, subfamily M, member 7, CHAK, CHAK1, LTRPC7, FLJ20117, TRP-PLIK, LTRPC ion channel family member 7, homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein FLJ20117, FLJ25718
Protein Accession Number:
NP_060142.3,NP_001288141.1
Protein Gi Number:
29893552
Ncbi Gene Id Number:
54822
Background Reference 1:
Nadler MJ, Hermosura MC, Inabe K, Perraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet JP, Penner R, Scharenberg AM, Fleig A. LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability. Nature 2001 May 31;411(6837):590-5

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-531

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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