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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRPV3 with DYKDDDDK tag probed with 46-915 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with 46-915 (1mg/ml) in Lane B. Prima
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRPV3 with DYKDDDDK tag probed with 46-915 (0.5ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with 46-915 (1mg/ml) in Lane B. Prima

TRPV3 Antibody

CATALOG NUMBER: 46-915

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-NKIQDSSRNNSK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:In transfected HEK293 transiently expressing full-length Human TRPV3 (myc and DYKDDDDK tagged), a band of approx. 90kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 0.5-1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRPV3
Additional Names:
TRPV3, transient receptor potential cation channel, subfamily V, member 3, VRL3, vanilloid receptor 3, vanilloid receptor-related osmotically activated channel protein
Protein Accession Number:
NP_659505.1
Protein Gi Number:
21735483
Ncbi Gene Id Number:
162514
Background Reference 1:
Xu H, Ramsey IS, Kotecha SA, Moran MM, Chong JA, Lawson D, Ge P, Lilly J, Silos-Santiago I, Xie Y, DiStefano PS, Curtis R, Clapham DE. TRPV3 is a calcium-permeable temperature-sensitive cation channel. Nature. 2002 Jul 11;418(6894):181-6. Epub 2002 Jun 23

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-915

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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