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46-533 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-533 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TRPV5 Antibody

CATALOG NUMBER: 46-533

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SHRGWEILRQNT
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3ug/ml.Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for human retinal pigment epithelium as described in the following paper: Kennedy et al, Mol Vis. 2010 Apr 14;16:665-75; PMID: 20405023.
Positive Control 1:
Cat. No. 1307 - Human Pancreas Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TRPV5
Additional Names:
TRPV5 , transient receptor potential cation channel, subfamily V, member 5 , CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3
Protein Accession Number:
NP_062815.2
Protein Gi Number:
17505200
Ncbi Gene Id Number:
56302
Background Reference 1:
van der Eerden BC, Hoenderop JG, de Vries TJ, Schoenmaker T, Buurman CJ, Uitterlinden AG, Pols HA, Bindels RJ, van Leeuwen JP. The epithelial Ca2+ channel TRPV5 is essential for proper osteoclastic bone resorption. Proc Natl Acad Sci USA. 2005 Nov 29;102(48):17507-12. Epub 2005 Nov 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-533

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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