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Tryptophan Hydroxylase (Phospho-Ser58) Antibody
Tryptophan Hydroxylase (Phospho-Ser58) Antibody

Tryptophan (phospho Ser58) Hydroxylase Antibody

CATALOG NUMBER: XPS-1030

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TPH (Ser58) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphoSer58 of TPH.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Tryptophan Hydroxylase antibody is specific immunolabeling of the ~55k TPH protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus.
Predicted Molecular Weight:
55
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Tph1
Protein Accession Number:
P09810
Protein Gi Number:
136122
Ncbi Gene Id Number:
24848
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.
Background Reference 1:
Martinez, A., Knappskog, P.M. and Haavik, J. "A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis," Curr. Med. Chem., 8 (2001) 1077 - 1091.
Background Reference 2:
Jiang, G.C., Yohrling, G.J., Schmitt, J.D. and Vrana, K.E., "Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling," J. Mol. Biol., 302 (2000) 1005 - 1017.
Background Reference 3:
Banik, U., Wang, G.A., Wagner, P.D., and Kaufman, S., "Interaction of phosphorylated tryptophan hydroxylase with 14-3-3 proteins," J. Biol. Chem. 272 (1997) 26219-26225.
Background Reference 4:
Kuhn, D.M., Arthur, Jr., R., and States, J.C., "Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A," J. Neurochem. 68 (1997) 2220 - 2223.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1030

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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