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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

TSFM Antibody

CATALOG NUMBER: 25-011

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TSFM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TSFM antibody can be used for detection of TSFM by ELISA at 1:312500. TSFM antibody can be used for detection of TSFM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
38 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TSFM antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TSFM
Additional Names:
TSFM, COXPD3, EF-TS, EF-Tsmt, EFTS, EFTSMT
Protein Accession Number:
NP_005717
Protein Gi Number:
171846268
Ncbi Gene Id Number:
10102
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The TSFM protein is a mitochondrial translation elongation factor. Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes.Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).
Background Reference 1:
Smeitink, J.A., (2006) Am. J. Hum. Genet. 79 (5), 869-877.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-011

List Size:
100 ul

List Price:

$519.00

Datasheet
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