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Western blot analysis of extracts of various cell lines, using TSG101 antibody (18-579) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug TSG101 antibody (18-579). Western blot was performed from the immunoprecipitate using TSG101 antibody (18-579) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TSG101 antibody (18-579) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

TSG101 Antibody

CATALOG NUMBER: 18-579

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TSG101 (NP_006283.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:200 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
MCF7
Positive Control 4:
K-562
Positive Control 5:
Mouse spinal cord
Positive Control 6:
Rat heart
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TSG101
Additional Names:
TSG101, TSG10, VPS23
Ncbi Gene Id Number:
7251
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-579

List Size:
100 uL

List Price:

$436.00

Datasheet
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