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Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TSHB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of TSHB Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

TSHB Antibody

CATALOG NUMBER: 63-549

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TSHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human TSHB.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSHB
Additional Names:
Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, TSH-B, TSH-beta, Thyrotropin beta chain, Thyrotropin alfa, TSHB
Protein Accession Number:
P01222
Protein Gi Number:
311033515
Ncbi Gene Id Number:
7252
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSHB is indispensable for the control of thyroid structure and metabolism.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-549

List Size:
400 ul

List Price:

$495.00

Datasheet
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