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IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone SPM223). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone SPM223). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

TSHR alpha chain Antibody [SPM223]

CATALOG NUMBER: 33-790

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human TSH Receptor alpha chain protein was used as the immunogen for this TSHR antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSHR antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TSHR
Additional Names:
TSHR, LGR3, MGC75129, hTSHR-I, CHNG1
Ncbi Gene Id Number:
7253
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-790

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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