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<strong>Figure 1 Western Blot Validation in Mouse A-20 Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: TSLP 4025 (A: 0.5 μg/mL, B: 1 μg/mL, C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Immunofluorescence Validation of TSLP in Mouse Brain Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling TSLP with 4025 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 3 Immunohistochemistry Validation of TSLP in Mouse Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using anti-TSLP antibody (4025) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Induction Validation of TSLP in Nasal Mucosa of Allergic Rhinitis (AR) mice (Wang et al., 2012)</strong><br> Western Blot images showing theTSLP protein detected by anti-mouse TSLP antibodies in normal mice (Control) or AR mice. TSLP protein expression levels were higher in AR mice and AR-GFP mice.
<strong>Figure 5 Induction Validation of TSLP in Rat VSMCs (Zhao et al., 2012)</strong><br> Immunohistochemical images showing the TSLP protein detected by anti-TSLP antibodies in Rat VSMCs without (Control) or with treatment with TNF-alpha, Ang II, Ang II+PDTC , Ang II +valsartan. TNF-α treatment is used as a positive control. The production of TSLP was increased after Ang II treatment.
Figure 1 Western Blot Validation in Mouse A-20 Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: TSLP 4025 (A: 0.5 μg/mL, B: 1 μg/mL, C: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

TSLP Antibody

CATALOG NUMBER: 4025

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Anti-TSLP antibody (4025) was raised against a peptide corresponding to 20 amino acids near the center of mouse TSLP.
The immunogen is located within the amino acids 70-120 of TSLP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 0.5 - 2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 16 kD
Observed: 24 kD (Post-modifications: 3 N-linked glycosylations)
Isoforms:
Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). 4025 can detect mouse and rat isoform.
Purification:
TSLP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TSLP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TSLP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSLP
Additional Names:
TSLP Antibody: Thymic stromal lymphopoietin
Protein Accession Number:
NP_067342
Protein Gi Number:
14719428
Ncbi Gene Id Number:
85480
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSLP Antibody: Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. At least two differentially spliced isoforms of TSLP are known to exist.
Background Reference 1:
Ziegler and Liu. Nature Immunol. 2006; 7:709-14.
Background Reference 2:
Sims et al. J. Exp. Med. 2000; 192:671-80.
Background Reference 3:
Levin et al. J. Immunol. 1999; 162:677-83.
Background Reference 4:
Quentmeier et al. Leukemia 2001; 15:1286-92.
Citation 1:
Wang et al. Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis. Exp Mol Med. 2012;44(3):177-90. PMID: PMID: 22170034.
Citation 2:
Zhao et al. Angiotensin II induces TSLP via an AT1 receptor/NF-KappaB pathway, promoting Th17 differentiation. Cell Physiol Biochem. 2012;30(6):1383-97. PMID: PMID: 23154814.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4025

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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