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Western blot analysis of TSPAN1 Antibody in mouse heart tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TSPAN1 Antibody in mouse heart tissue lysates (35ug/lane)

TSPAN1 Antibody

CATALOG NUMBER: 63-594

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This TSPAN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids from the Central region of human TSPAN1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSPAN1
Additional Names:
Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1
Protein Accession Number:
O60635
Ncbi Gene Id Number:
10103
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSPAN1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
Background Reference 1:
Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009)
Background Reference 2:
Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-594

List Size:
400 ul

List Price:

$495.00

Datasheet
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