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Western blot analysis of TSPAN12 Antibody in T47D cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of TSPAN12 Antibody in T47D cell line lysates (35ug/lane)

TSPAN12 Antibody

CATALOG NUMBER: 63-593

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This TSPAN12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-259 amino acids from the C-terminal region of human TSPAN12.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSPAN12
Additional Names:
Tetraspanin-12, Tspan-12, Tetraspan NET-2, Transmembrane 4 superfamily member 12, TSPAN12, NET2, TM4SF12
Protein Accession Number:
O95859
Ncbi Gene Id Number:
23554
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
Background Reference 1:
Xu,D., et.al., FASEB J. 23 (11), 3674-3681 (2009)
Background Reference 2:
Berditchevski,F.et.al., J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-593

List Size:
400 ul

List Price:

$495.00

Datasheet
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