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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM,K562,HL-60 cell line lysates (35ug/lane).
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

TSPAN2 Antibody

CATALOG NUMBER: 63-894

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TSPAN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human TSPAN2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSPAN2
Additional Names:
Tetraspanin-2, Tspan-2, Tetraspan NET-3, TSPAN2
Protein Accession Number:
O60636
Ncbi Gene Id Number:
10100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSPAN2 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.
Background Reference 1:
Davila,S., et.al., Genes Immun. 11 (3), 232-238 (2010)
Background Reference 2:
Hemler,M.E. J. Cell Biol. 155 (7), 1103-1107 (2001)
Background Reference 3:
Berditchevski,F. J. Cell. Sci. 114 (PT 23), 4143-4151 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-894

List Size:
400 ul

List Price:

$495.00

Datasheet
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