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Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected TSPAN3 protein (arrow).
TSPAN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected TSPAN3 protein (arrow).

TSPAN3 Antibody

CATALOG NUMBER: 61-974

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TSPAN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human TSPAN3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSPAN3
Additional Names:
Tetraspanin-3, Tspan-3, Tetraspanin TM4-A, Transmembrane 4 superfamily member 8, TSPAN3, TM4SF8
Protein Accession Number:
O60637
Ncbi Gene Id Number:
10099
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thetransmembrane 4 superfamily, also known as the tetraspanin family.Most of these members are cell-surface proteins that arecharacterized by the presence of four hydrophobic domains. Theproteins mediate signal transduction events that play a role in theregulation of cell development, activation, growth and motility.The use of alternate polyadenylation sites has been found for thisgene.
Background Reference 1:
Berditchevski, F. J. Cell. Sci. 114 (PT 23), 4143-4151 (2001) :
Background Reference 2:
Todd, S.C., et al. Biochim. Biophys. Acta 1399(1):101-104(1998)
Background Reference 3:
Wu, Y.J., et al. J. Biol. Chem. 266(26):17566-17572(1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-974

List Size:
400 ul

List Price:

$495.00

Datasheet
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