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46-538 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-538 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-538 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-538 (0.1ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TSPO Antibody

CATALOG NUMBER: 46-538

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RDNHGWRGGRRLPE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Breast Cancer lysates (calculated MW of 18.8kDa according to NP_000705.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Colon shows strong cytoplasm staining of absorptive cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise one isoform (NP_000705.2) only.
Positive Control 1:
Cat. No. 1323 - Human Breast Tumor Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TSPO
Additional Names:
TSPO, PBR, translocator protein (18kDa) , BZRP, DBI, IBP, MBR, PKBS, PTBR, mDRC, pk18 , OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine receptor, peripheral-type benzodiazepine receptor
Protein Accession Number:
NP_000705.2
Protein Gi Number:
74275350
Ncbi Gene Id Number:
706
Background Reference 1:
Papadopoulos V, Baraldi M, Guilarte TR, Knudsen TB, Lacapere JJ, Lindemann P, Norenberg MD, Nutt D, Weizman A, Zhang MR, Gavish M. Translocator protein (18 kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function. Trends Pharmacol Sci. 2006 Aug;27(8):402-9. Epub 2006 Jul 5. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-538

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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