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Western blot analysis of extracts of various cell lines, using TSPO antibody (13-790).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse kidney using TSPO antibody (13-790) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TSPO antibody (13-790) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using TSPO antibody (13-790) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using TSPO antibody (13-790) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SW480 cells using TSPO antibody (13-790).
Immunofluorescence analysis of HeLa cells using TSPO antibody (13-790).
Immunofluorescence analysis of PC-3M cells using TSPO antibody (13-790).
Western blot analysis of extracts of various cell lines, using TSPO antibody (13-790).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

TSPO Antibody [H2F7]

CATALOG NUMBER: 13-790

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human TSPO
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:5000 - 1:10000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
293T
Positive Control 2:
NIH/3T3
Positive Control 3:
HepG2
Predicted Molecular Weight:
Observed: 15kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TSPO
Additional Names:
TSPO, translocator protein (18 kDa), BZRP, DBI, IBP, MBR, PBR, PKBS, PTBR, mDRC, pk18, OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine receptor, peripheral-type benzodiazepine receptor
Ncbi Gene Id Number:
706
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-790

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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