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Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (14-295) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug TSPYL1 antibody (14-295). Western blot was performed from the immunoprecipitate using TSPYL1 antibody (14-295) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (14-295) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

TSPYL1 Antibody

CATALOG NUMBER: 14-295

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TSPYL1 (NP_003300.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:100
Positive Control 1:
U-87MG
Positive Control 2:
SKOV3
Positive Control 3:
LO2
Positive Control 4:
HeLa
Positive Control 5:
293T
Positive Control 6:
A-549
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TSPYL1
Additional Names:
Testis-specific Y-encoded-like protein 1, TSPY-like protein 1, TSPYL1, TSPYL
Ncbi Gene Id Number:
7259
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-295

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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