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Western blot analysis of extracts of various cell lines, using TSPYL2 antibody (14-801) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using TSPYL2 antibody (14-801) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TSPYL2 Antibody

CATALOG NUMBER: 14-801

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 240-460 of human TSPYL2 (NP_071400.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:1000 - 1:2000
Positive Control 1:
LO2
Positive Control 2:
HeLa
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse testis
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TSPYL2
Additional Names:
testis-specific protein Y encoded-like 2, testis-specific Y-encoded-like protein 2, nuclear protein of 79 kDa, differentially-expressed nucleolar TGF-beta1 target protein, differentially expressed nucleolar TGF-beta1 target, cutaneous T-cell lymphoma-associated tumor antigen se20-4, cutaneous T-cell lymphoma-associated antigen se20-4, cell division autoantigen 1, TSPY-like protein 2, CTCL-associated antigen se20-4, CTCL tumor antigen se20-4, CASK-interacting nucleosome assembly protein, TSPX, SE204, NP79, HRIHFB2216, DENTT, CTCL, CINAP, CDA1, RP1-290F12.2, TSPY-like 2, TSPYL2
Ncbi Gene Id Number:
64061
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the testis-specific protein Y-encoded, TSPY-like/SET/nucleosome assembly protein-1 superfamily. The encoded protein is localized to the nucleolus where it functions in chromatin remodeling and as an inhibitor of cell-cycle progression. This protein may play a role in the suppression of tumor growth.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-801

List Size:
100 uL

List Price:

$436.00

Datasheet
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