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Western blot analysis of extracts of various cell lines, using TST antibody (13-709) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (13-709). Western blot was performed from the immunoprecipitate using TST antibody (13-709) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TST antibody (13-709) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

TST Antibody

CATALOG NUMBER: 13-709

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:1000 - 1:2000
IP: 1:50 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
DU145
Positive Control 3:
MCF-7
Positive Control 4:
293T
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TST
Additional Names:
TST, MGC19578, RDS
Ncbi Gene Id Number:
7263
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-709

List Size:
100 uL

List Price:

$436.00

Datasheet
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