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Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 μg/mL.
Immunohistochemistry of TTBK1 in mouse brain tissue with TTBK1 antibody at 2.5 μg/mL.
Immunofluorescence of TTBK1 in Human Brain tissue with TTBK1 antibody at 20 μg/mL.
Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 μg/mL.
Trial Size

TTBK1 Antibody

CATALOG NUMBER: 5013

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TTBK1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TTBK1.
The immunogen is located within amino acids 1190 - 1240 of TTBK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 145, 151 kDa
Observed: 140 kDa
Purification:
TTBK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TTBK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TTBK1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TTBK1
Additional Names:
TTBK1 Antibody: BDTK, BDTK, KIAA1855, Tau-tubulin kinase 1, Brain-derived tau kinase
Protein Accession Number:
Q5TCY1
Protein Gi Number:
97203020
Ncbi Gene Id Number:
84630
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TTBK1 Antibody: Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients.
Background Reference 1:
Sato S, Cerny RL, Bueschner JL, et al. Tau-tubulin kinase 1 (TTBK1), a neuron-specific tau kinase candidate, is involved in tau phosphorylation and aggregation. J. Neurochem. 2006; 98:1573-84.
Background Reference 2:
Sato S, Xu J, Okuyama S, et al. Spatial learning impairment, enhanced CDK5/p35 activity, and downregulation of NMDA receptor expression in transgenic mice expressing tau-tubulin kinase 1. J. Neurosci. 2008; 28:14511-21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5013

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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