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Western blot analysis of extracts of various cell lines, using TUBB3 antibody (18-304) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using TUBB3 antibody (18-304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TUBB3 Antibody [5D5D2]

CATALOG NUMBER: 18-304

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human TUBB3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
SH-SY5Y
Positive Control 2:
MCF7
Positive Control 3:
HeLa
Positive Control 4:
293T
Positive Control 5:
mouse testis
Positive Control 6:
mouse eye
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TUBB3
Additional Names:
beta-4, CDCBM, CFEOM3A, class III beta-tubulin, OTTHUMP00000176846, TUBB3, TUBB4, tubulin beta-3 chain, tubulin beta-4 chain, tubulin beta-III, tubulin, beta 3
Ncbi Gene Id Number:
10381
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-304

List Size:
100 uL

List Price:

$540.00

Datasheet
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