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Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse brain tissue lysates (15ug/lane).
Western blot analysis in HepG2 cell line lysates (15ug/lane).
TUBB3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

TUBB3 Antibody

CATALOG NUMBER: 62-035

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Monkey, Chicken, Bovine
Immunogen:
This TUBB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human TUBB3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TUBB3
Additional Names:
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB3, TUBB4
Protein Accession Number:
Q13509
Protein Gi Number:
20455526
Ncbi Gene Id Number:
10381
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
Background Reference 1:
Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996)
Background Reference 2:
Khan, I.A., et al. Biochemistry 35(12):3704-3711(1996)
Background Reference 3:
Vinores, S.A., et al. Exp. Eye Res. 60(4):385-400(1995)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-035

List Size:
400 ul

List Price:

$495.00

Datasheet
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