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Western blot analysis of extracts of various cell lines, using TUFM antibody (22-209) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody (22-209) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using TUFM antibody (22-209). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody (22-209). Western blot was performed from the immunoprecipitate using TUFM antibody (22-209) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TUFM antibody (22-209) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TUFM Antibody

CATALOG NUMBER: 22-209

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
HepG2
Positive Control 3:
HeLa
Positive Control 4:
A-431
Positive Control 5:
Jurkat
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TUFM
Additional Names:
TUFM, COXPD4, EF-TuMT, EFTU, P43
Ncbi Gene Id Number:
7284
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-209

List Size:
100 uL

List Price:

$436.00

Datasheet
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