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Western blot analysis using Mouse TUG monoclonal antibody against NIH / 3T3 cell lysate.
Western blot analysis using Mouse TUG monoclonal antibody against NIH / 3T3 cell lysate.

TUG Antibody [4A11A6G11]

CATALOG NUMBER: 32-195

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Mouse
Immunogen:
Ni-NTA purified truncated recombinant TUG expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Mouse TUG monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ASPSCR1
Additional Names:
ASPL, RCC17, TUG, UBXD9, UBXN9, Alveolar soft part sarcoma chromosomal region candidate gene 1 proteinTUG, ASPL, ASPS, ASPCR1
Protein Accession Number:
Q9BZE9
Protein Gi Number:
74717746
Ncbi Gene Id Number:
79058
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.
Background Reference 1:
Nature 2003,425:727 - 733.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-195

List Size:
0.1 mL

List Price:

$408.00

Datasheet
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