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46-721 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-721 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TUSC3 Antibody

CATALOG NUMBER: 46-721

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: PQRQCSVCRQAN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 39.7kDa according to NP_006756.2 and of 39.6kDa according to NP_839952.1). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Specificity:
This antibody is expected to recognise both reported isoforms (NP_006756.2; NP_839952.1).
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TUSC3
Additional Names:
TUSC3, tumor suppressor candidate 3, D8S1992, MGC13453, N33, OST3A, Putative prostate cancer tumor suppressor
Protein Accession Number:
NP_006756.2,NP_839952.1
Protein Gi Number:
30410788
Ncbi Gene Id Number:
7991
Background Reference 1:
Pils D, Horak P, Gleiss A, Sax C, Fabjani G, Moebus VJ, Zielinski C, Reinthaller A, Zeillinger R, Krainer M. Five genes from chromosomal band 8p22 are significantly down-regulated in ovarian carcinoma: N33 and EFA6R have a potential impact on overall survival. Cancer. 2005 Dec 1;104(11):2417-29.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-721

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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