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Western blot analysis of extracts of various cell lines, using TWF2 antibody (15-123) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug TWF2 antibody (15-123). Western blot was performed from the immunoprecipitate using TWF2 antibody (15-123) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TWF2 antibody (15-123) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.

TWF2 Antibody

CATALOG NUMBER: 15-123

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human TWF2 (NP_009215.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Jurkat
Positive Control 2:
293T
Positive Control 3:
A-431
Positive Control 4:
Mouse heart
Positive Control 5:
Mouse thymus
Predicted Molecular Weight:
Observed: 40kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TWF2
Additional Names:
Twinfilin-2, A6-related protein, hA6RP, Protein tyrosine kinase 9-like, Twinfilin-1-like protein, TWF2, PTK9L
Ncbi Gene Id Number:
11344
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-123

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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