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Western blot analysis of lysed extracts from JK, Raji and HL-60 cells using Twist1 Antibody.
Western blot analysis of lysed extracts from JK, Raji and HL-60 cells using Twist1 Antibody.

Twist1 Antibody

CATALOG NUMBER: 79-743

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Twist1 antibody was raised against a peptide sequence around aa.18~22 (S-N-S-E-E) derived from Human Twist1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous levels of total Twist1 protein.
Predicted Molecular Weight:
28 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
TWIST1
Additional Names:
CRS, SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38
Protein Accession Number:
NP_000465.1
Protein Gi Number:
4507741
Ncbi Gene Id Number:
7291
Background:
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
Background Reference 1:
"Twist regulates cytokine gene expression through a negative feedback loop that represses NF-kappaB activity. "Sosic D., Richardson J. A., Yu K., Ornitz D. M., Olson E. N. Cell 112:169-180 (2003) "Mutations of the TWIST gene in the Saethre-Chotzen syndrome. "el Ghouzzi V., le Merrer M., Perrin-Schmitt F., et al., Nat. Genet. 15:42-46 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-743

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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