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Western blot analysis of extracts of various cell lines, using TXN antibody (15-733) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using TXN antibody (15-733) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using NFE2L1 antibody (15-708) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TXN antibody (15-733) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TXN Antibody

CATALOG NUMBER: 15-733

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human TXN (NP_003320.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TXN
Additional Names:
TXN, ADF, ATL-derived factor, SASP, Thioredoxin, TRX1, Thioredoxin delta 3, TRX, TXN delta 3, TRDX
Ncbi Gene Id Number:
7295
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-733

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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