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Western blot analysis of extracts of various cell lines, using TXN2 antibody (14-528) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using TXN2 antibody (14-528) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TXN2 antibody (14-528) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using TXN2 antibody (14-528) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TXN2 antibody (14-528) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TXN2 antibody (14-528). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TXN2 antibody (14-528) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

TXN2 Antibody

CATALOG NUMBER: 14-528

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human TXN2 (NP_036605.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Raji
Positive Control 2:
293T
Positive Control 3:
SW620
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse brain
Positive Control 6:
Mouse bone marrow
Predicted Molecular Weight:
Observed: 13kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TXN2
Additional Names:
Thioredoxin, mitochondrial, MTRX, Mt-Trx, Thioredoxin-2, TXN2, TRX2
Ncbi Gene Id Number:
25828
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-528

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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