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42-369 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-369 (0.1ug/ml) staining of Mouse Lymph Nodes (A) and Mouse Intestine (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-369 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.
42-369 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TXNDC5 Antibody

CATALOG NUMBER: 42-369

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-RDLESLREYVESQLQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa plus faint 35kDa bands observed in Human Lymph Nodes lysates and 48kDa in Mouse Lymph Node and in Mouse Intestine lysates (calculated MW of 47.6kDa according to Human NP_110437.2, 46.4kDa to Mouse NP_663342.3) and of 36.2kDa according to Human NP_001139021.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).
Positive Control 1:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TXNDC5
Additional Names:
TXNDC5, thioredoxin domain containing 5 (endoplasmic reticulum), ERP46, EndoPDI, Hcc-2, MGC3178, PDIA15, UNQ364, ER protein 46, endoplasmic reticulum protein ERp46, endoplasmic reticulum resident protein 46, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain-containing protein 5, thioredoxin related protein, thioredoxin-like protein p46
Protein Accession Number:
NP_110437.2,NP_001139021.1
Protein Gi Number:
42794771
Ncbi Gene Id Number:
81567

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-369

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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