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42-430 (0.05ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-430 (0.1ug/ml) staining of Mouse Lymph Nodes (A), Stomach (B) and Intestine (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-430 (3.75ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-430 (0.05ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TXNDC5 Antibody

CATALOG NUMBER: 42-430

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-RDGKKVDQYKGKRD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50+38kDa bands observed in Human Lymph Nodes lysates (calculated MW of 47.6kDa according to NP_110437.2 and of 36.2kDa according to NP_001139021.1). A 48kDa band was observed in Mouse Lymph Node while a 37-38kDa doublet was observed n Mouse Stomach lysates and in Mouse Intestine lysates. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_110437.2; NP_001139021.1).
Positive Control 1:
Cat. No. 1369 - Human Lymph node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TXNDC5
Additional Names:
TXNDC5, thioredoxin domain containing 5 (endoplasmic reticulum), ERP46, EndoPDI, Hcc-2, MGC3178, PDIA15, UNQ364, ER protein 46, endoplasmic reticulum protein ERp46, endoplasmic reticulum resident protein 46, endothelial protein disulphide isomerase, protein disulfide isomerase family A, member 15, thioredoxin domain-containing protein 5, thioredoxin related protein, thioredoxin-like protein p46
Protein Accession Number:
NP_110437.2,NP_001139021.1
Protein Gi Number:
42794771
Ncbi Gene Id Number:
81567

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-430

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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