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Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Raji whole cell lysate Lysates/proteins at 20 ug per lane.

TXNL2 Antibody

CATALOG NUMBER: 56-228

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TXNL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the N-terminal region of human TXNL2.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GLRX3
Additional Names:
Glutaredoxin-3, PKC-interacting cousin of thioredoxin, PICOT, PKC-theta-interacting protein, PKCq-interacting protein, Thioredoxin-like protein 2, GLRX3, PICOT, TXNL2
Protein Accession Number:
O76003
Ncbi Gene Id Number:
10539
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GLRX3 is a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (By similarity). May play a role in regulating the function of the thioredoxin system. Does not posses any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.
Background Reference 1:
Ohayon, A., et al. J. Histochem. Cytochem. 58(9):799-806(2010)
Background Reference 2:
Haunhorst, P., et al. Biochem. Biophys. Res. Commun. 394(2):372-376(2010)
Background Reference 3:
Ohayon, A., et al.Immunotoxicol 7(1):8-14(2010)
Background Reference 4:
Cha, M.K., et al. Cancer Epidemiol 33 (3-4), 281-287 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-228

List Size:
400 ul

List Price:

$495.00

Datasheet
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