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Western blot analysis of Tyrosinase antibody in HepG2 cell line lysates (35ug/lane)
Tyrosinase Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Tyrosinase Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Tyrosinase antibody in HepG2 cell line lysates (35ug/lane)

Tyrosinase Antibody

CATALOG NUMBER: 62-843

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Tyrosinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-513 amino acids from the C-terminal region of human Tyrosinase.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
60 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TYR
Additional Names:
Tyrosinase, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
Protein Accession Number:
P14679
Protein Gi Number:
401235
Ncbi Gene Id Number:
7299
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The protein has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this protein result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation.
Background Reference 1:
Ostankovitch,M. J. Immunol. 182 (8), 4830-4835 (2009)
Background Reference 2:
Chintamaneni,C.D. Proc. Natl. Acad. Sci. U.S.A. 88 (12), 5272-5276 (1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-843

List Size:
400 ul

List Price:

$495.00

Datasheet
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